Application

Anti-Human IgG3 antibody, mouse monoclonal has been used in indirect ELISA and immunoprecipitation.

Biochem/physiol Actions

IgG1 and IgG3 are the only subclasses capable of adherence to mononuclear phagocytes and are recognized readily by the Fc receptors on various reticulo-endothelial . IgG3 deficiency has been associated with a history of recurrent infectious, leading to chronic lung disease.

General description

Human IgG consist of four subclasses (1-4) that can be recognized by antigenic differences in their heavy chains. They constitute approximately 65, 30, 5 and 4% of the total IgG, respectively. The abundance of the different IgG subclasses in the bloodstream varies with age, IgG1 and IgG3 reach normal adult levels by 5-7 years of age while IgG2 and IgG4 levels rise more slowly, reaching adult levels at about 10 years of age.

Immunogen

Human IgG3 myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.